Phytochemical analysis of nine Hypericum L. species from Serbia and the F.Y.R. Macedonia

The methanol extracts of the aerial parts of nine Hypericum species (H. barbatum, H. hirsutum, H. linarioides, H. maculatum, H. olympicum, H. perforatum, H. richeri, H. rumeliacum and H. tetrapterum), collected on different locations in Serbia and the F.Y.R. Macedonia, were obtained by accelerated solvent extraction (ASE) and analyzed for the content of four constituents (hyperoside, quercitrin, hyperforin and hypericin) by LC-MS/ MS. All studied extracts contained the characteristic four constituents, but their contents varied between different species and locations. The content of hypericin in H. barbatum was significantly higher (3.9 times) than that in H. perforatum.