Related Experiment Video
Updated: Aug 9, 2026

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
Published on: May 4, 2018
On the effect of a point mutation on the reactivity of CuZn superoxide dismutase: a theoretical study
Andrea Amadei1, Maira D'Alessandro, Maurizio Paci
1Dipartimento di Scienze e Tecnologie Chimiche Università di Roma "Tor Vergata", Via della Ricerca Scientifica 1, 00133 Roma, Italy. andrea.amadei@uniroma2.it
Abstract:
In this paper, we investigate the effects of a point mutation on the enzymatic activity of copper-zinc superoxide dismutase, which we recently studied in detail by means of a theoretical-computational procedure. Comparison of the reactivity of the initial catalytic steps in this mutant (G93A mutation far from the active site) with our previous data, reveals the beautiful mechanical-dynamical architecture of the enzyme, altered by such an apparently irrelevant mutation. Finally, our results suggest a possible atomic-molecular-based explanation for the mutant-pathology correlation, in line with the most recent experimental data.
More Related Videos
07:16Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
19:16The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Related Concept Videos
Spontaneous and Induced Mutations
Ligand Binding and Linkage
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein.
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Mutations
Bioactivation and Tissue Toxicity