Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pyrosequencing protocol requiring a unique biotinylated primer.

Jose Luis Royo1, Manuel Hidalgo Pascual, Ana Salinas

  • 1Departamento de Genomica Estructural, Neocodex SL, Sevilla, Spain. jlroyo@neocodex.es

Clinical Chemistry and Laboratory Medicine
|April 8, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human IL-34 Deficiency Primes Microglia Toward Alzheimer's Disease-Associated States.

medRxiv : the preprint server for health sciences·2026
Same author

Endoscopic negative-pressure therapy for anastomotic leaks after upper gastrointestinal surgery: systematic review and meta-analysis.

Surgical endoscopy·2026
Same author

Landscape of copy number variants in Spanish people with dementia.

NPJ genomic medicine·2026
Same author

Listening for Alzheimer's clues: machine learning analysis of multidomain speech features for cognitive impairment screening.

Frontiers in aging neuroscience·2026
Same author

Non-linear associations between sleep duration and plasma p-tau181 in the Framingham Heart Study.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Low overlap of plasma and CSF protein quantitative trait loci affects protein discovery for neurological disease.

Science translational medicine·2026
Same journal

Assessing neonatal and paediatric acute kidney injury: current practices and insights from the IFCC C-ETPLM International Laboratory Survey.

Clinical chemistry and laboratory medicine·2026
Same journal

A novel comprehensive non-invasive prenatal testing system for the simultaneous detection of single-gene disorders, chromosomal structural rearrangements, and aneuploidy.

Clinical chemistry and laboratory medicine·2026
Same journal

Fish-shaped erythrocytes and pincer cells co-occur in distinct hematological disorders and are associated with anemia severity.

Clinical chemistry and laboratory medicine·2026
Same journal

Artificial general intelligence and the clinical laboratory: a paradigm shift toward Lab 2.0.

Clinical chemistry and laboratory medicine·2026
Same journal

Addressing property and unit discordance in laboratory interoperability: the role of NPU as a governance layer.

Clinical chemistry and laboratory medicine·2026
Same journal

Ethical evaluation of reflex and reflective testing in conformity with ISO 15189:2022 at the value-based laboratory medicine era.

Clinical chemistry and laboratory medicine·2026
See all related articles

Researchers developed a new pyrosequencing method using a single universal biotinylated primer for genotyping multiple single-nucleotide polymorphisms (SNPs). This streamlines high-throughput analysis of genetic variants in structural genomics.

Area of Science:

  • Genomics
  • Molecular Biology

Background:

  • DNA sequencing advances have generated millions of human genome polymorphisms requiring validation.
  • High-throughput analysis of genetic variants is crucial for structural genomics.
  • Standard pyrosequencing requires specific labeled primers for each polymorphism.

Purpose of the Study:

  • To develop a more efficient pyrosequencing method.
  • To reduce the need for numerous specific labeled primers.

Main Methods:

  • Utilized adjusted primer stoichiometry to standardize amplicon labeling.
  • Employed a single biotinylated universal primer (BM13S) for all amplicons.

Main Results:

  • Successfully circumvented the requirement for individual biotinylated primers per single-nucleotide polymorphism (SNP).

Related Experiment Videos

  • Demonstrated the protocol's efficacy by genotyping three SNPs (calpain-10, caveolin-1, CYP19A1).

    • Conclusions:

    • The novel approach offers an alternative to standard pyrosequencing protocols.
    • A single biotinylated primer is sufficient for genotyping various SNPs, simplifying the process.