Related Experiment Video
Updated: Aug 9, 2026
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Review of successful treatment for Helicobacter species in laboratory mice
1Central Biomedical Services, Imperial College, London, UK. a.kerton@imperial.ac.uk
Abstract:
Three variations of the amoxycillin-based triple therapy (amoxycillin, metronidazole and bismuth) were administered in the diet, by oral gavage or in the diet in conjunction with cross-fostering on to Helicobacter-free foster mothers to mice naturally infected with H. hepaticus and/or H. bilis. The presence of Helicobacter species was determined by polymerase chain reaction (PCR) analysis of faecal pellets. Helicobacter infection was eliminated in 50% of strains of mice treated by oral gavage; 57% of strains of mice treated by medicated diet alone and 100% of strains of mice treated with the medicated diet in conjunction with cross-fostering on to Helicobacter-free foster mothers. Eight strains of mice were successfully treated for Helicobacter infection over a two-year period. The mouse colony has been maintained Helicobacter free, as determined by PCR analysis and has remained off treatment from December 2002 to March 2005.
Insights
Amoxycillin-based triple therapy effectively eradicated Helicobacter infections in mice. Combining a medicated diet with cross-fostering achieved 100% eradication, maintaining a Helicobacter-free colony.
Area of Science:
- Veterinary Medicine
- Microbiology
- Animal Health
Background:
- Naturally occurring Helicobacter hepaticus and/or H. bilis infections pose challenges in mouse colonies.
- Effective eradication strategies are crucial for maintaining research integrity.
Purpose of the Study:
- To evaluate the efficacy of amoxycillin-based triple therapy in eliminating Helicobacter infections in mice.
- To compare different administration methods for the therapeutic regimen.
Main Methods:
- Three variations of amoxycillin-based triple therapy (amoxycillin, metronidazole, bismuth) were tested.
- Administration routes included medicated diet, oral gavage, and medicated diet with cross-fostering.
- Helicobacter presence was confirmed via polymerase chain reaction (PCR) analysis of fecal pellets.
Main Results:
- Oral gavage eliminated Helicobacter in 50% of mouse strains.
- Medicated diet alone eradicated infection in 57% of strains.
- Medicated diet combined with cross-fostering achieved 100% eradication.
Conclusions:
- Amoxycillin-based triple therapy, particularly when combined with cross-fostering, is highly effective for Helicobacter eradication in mice.
- Successful treatment over two years maintained a Helicobacter-free mouse colony.
- This approach ensures the long-term health and research reliability of mouse models.
More Related Videos
09:01Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
Published on: March 10, 2015
06:12Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model
Published on: May 29, 2015