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Updated: Aug 9, 2026

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
Published on: September 18, 2014
Enhancement of transcriptional activity of DNA complexes by amphoteric PEG derivative
Yoshiyuki Koyama1, Misa Yamashita, Naoko Iida-Tanaka
1Department of Textile Science and Department of Food Science, Otsuma Women's University, Tokyo, 102-8357 Japan. koyama@otsuma.ac.jp
Abstract:
A water-soluble PEG derivative having both amino and carboxylic acid side chains (PEG-AC) was synthesized and explored for its transcription- and transfection-enhancing activity. PEG-AC could be deposited onto the surface of DNA/polycation complexes to form a ternary complex with slightly negative surface potential. PEG-AC-coating on the plasmid/PEI complexes obviously enhanced their transcriptional activity, and 31-fold higher consumption of UTP was observed. Amphoteric PEG-AC would loosen the tightly compacted DNA/PEI complex and facilitate the approach of transcriptional factors. PEG-AC also evidently improved the transgene expression level on the cultured CHO cells.
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