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Primary cultures of guinea-pig gall-bladder epithelial cells suitable for patch-clamp studies
J A O'Brien1, P S James, M Torre
1Department of Cell Biology, AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge.
Experimental Physiology
|September 1, 1991
Abstract:
Cells derived from explants of guinea-pig gall-bladder maintained in culture for up to 14 days, exhibit a network of cytoskeletal filaments that are labelled by an anti-cytokeratin antibody, but not by an anti-desmin antibody, suggesting that they are of epithelial origin. These cells proved suitable for patch-clamp studies and by using this method channels selective for K+ and Cl- were observed.