Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DM-nitrophen AM is caged magnesium.

Graham C R Ellis-Davies1

  • 1Department of Pharmacology and Physiology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, USA. ged@drexel.edu

Cell Calcium
|April 11, 2006
PubMed
Summary

DM-nitrophen, a photolabile EDTA derivative, can serve as a caged calcium (Ca2+) or caged magnesium (Mg2+) ion. For intracellular applications, EGTA and BAPTA-based Ca2+ cages are recommended for photorelease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geometric principles of dendritic integration of excitation and inhibition in cortical neurons.

Science advances·2026
Same author

Geometric principles of dendritic integration of excitation and inhibition in cortical neurons.

bioRxiv : the preprint server for biology·2025
Same author

Abraham Patchornik: The Contemporary Relevance of His Work for Chemistry and Biology.

JACS Au·2025
Same author

Ca<sup>2+</sup>-dependent phosphodiesterase 1 regulates the plasticity of striatal spiny projection neuron glutamatergic synapses.

Cell reports·2024
Same author

Ca <sup>2+</sup> -dependent phosphodiesterase 1 regulates the plasticity of striatal spiny projection neuron glutamatergic synapses.

bioRxiv : the preprint server for biology·2024
Same author

Bidirectional Neuronal Actuation by Uncaging with Violet and Green Light.

Angewandte Chemie (International ed. in English)·2024

Area of Science:

  • Biochemistry
  • Cell Biology
  • Photochemistry

Background:

  • DM-nitrophen is a photolabile derivative of EDTA.
  • It functions as a caged calcium (Ca2+) or caged magnesium (Mg2+) ion.
  • Recent studies utilize the AM ester form of DM-nitrophen for loading intact cells, where it acts as a caged Mg2+.

Purpose of the Study:

  • To provide a historical overview of caged Ca2+ probe development and application.
  • To discuss the suitability of DM-nitrophen as a caged Ca2+ or caged Mg2+ probe.
  • To recommend preferred Ca2+ cages for intracellular photorelease.

Main Methods:

  • Review of published literature on caged Ca2+ probes.
  • Analysis of DM-nitrophen's properties and applications.
  • Comparison of different Ca2+ cage structures (EGTA, BAPTA based).

Main Results:

  • DM-nitrophen functions as both a caged Ca2+ and caged Mg2+ probe.
  • When loaded into cells using the AM ester, DM-nitrophen acts as a caged Mg2+.
  • EGTA and BAPTA-based Ca2+ cages are highlighted as superior for photoreleasing Ca2+ under physiological intracellular conditions.

Conclusions:

  • DM-nitrophen is a versatile photolabile compound with applications as both a caged Ca2+ and caged Mg2+.
  • The choice of Ca2+ cage is critical for effective photorelease in cellular environments.
  • EGTA and BAPTA derivatives offer advantages for controlled intracellular Ca2+ release studies.

Related Experiment Videos