Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method for chorioretinal oxygen tension measurement.

Mahnaz Shahidi1, Akbar Shakoor, Norman P Blair

  • 1Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois 60612, USA. mahnshah@uic.edu

Current Eye Research
|April 11, 2006
PubMed
Summary

A new optical imaging system measures oxygen tension (pO2) in retinal and choroidal blood vessels. This system successfully differentiated pO2 changes in these distinct vascular layers in rats, aiding research into eye oxygen dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Five-Year Risks of Systemic Complications with Biologic versus Conventional Therapy in Non-infectious Uveitis.

Ophthalmology·2026
Same author

Racial Variations in Retinal Oxygen Metabolic Biomarkers in Diabetic Retinopathy: A Pilot Study.

Journal of ophthalmology·2026
Same author

Association of papilledema severity with retinal vessel tortuosity in idiopathic intracranial hypertension.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2026
Same author

Glucagon-Like Peptide-1 Receptor Agonists and the Risk of Thyroid Eye Disease.

Ophthalmic plastic and reconstructive surgery·2026
Same author

Diagnosis of High Intracranial Pressure by Non-Optic Nerve Retinal Image Features.

Translational vision science & technology·2026
Same author

Retinal Hypoxia: Relation to Blood Flow and Oxygen Metabolic Biomarkers.

Investigative ophthalmology & visual science·2026

Area of Science:

  • Ophthalmology
  • Physiology
  • Biomedical Engineering

Background:

  • Oxygen tension (pO2) is critical for retinal and choroidal function.
  • Understanding localized pO2 dynamics is essential for diagnosing and treating various ocular diseases.
  • Existing methods for measuring chorioretinal pO2 have limitations in spatial resolution and specificity.

Purpose of the Study:

  • To develop and validate an optical imaging system for quantitative measurement of pO2 in the chorioretinal vasculature.
  • To assess the system's feasibility in distinguishing pO2 changes in retinal versus choroidal layers.
  • To investigate the role of nitric oxide in regulating chorioretinal oxygen tension.

Main Methods:

  • An optical section phosphorescence imaging system was adapted for quantitative pO2 measurements.

Related Experiment Videos

  • Intravenous injection of an oxygen-sensitive probe (Pd-porphyrin) and laser projection were used.
  • Frequency-domain analysis measured phosphorescence lifetime to determine pO2.
  • Experiments involved varying inspired oxygen and inhibiting nitric oxide synthase with Nomega-nitro-L-arginine (Nomega-NLA) in rat eyes.

    • Main Results:

    • System accurately measured systemic arterial pO2 and chorioretinal pO2.
    • Retinal and choroidal pO2 increased with elevated inspired oxygen.
    • Choroidal pO2 decreased significantly during Nomega-NLA infusion, while retinal pO2 remained stable.
    • Choroidal pO2 reduction was dose-dependent on Nomega-NLA infusion rate.

    Conclusions:

    • An effective optical method combining phosphorescence imaging and optical sectioning for chorioretinal pO2 measurement was established.
    • This technique offers potential for advancing the understanding of oxygen dynamics in normal and diseased eyes.
    • The findings highlight the differential regulation of oxygen tension in retinal and choroidal vasculatures.