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Experimental glaucoma significantly decreases atrial natriuretic factor (ANF) receptors in the ciliary processes of
R Fernandez-Durango1, J M Ramirez, A Trivino
1Departamento de Medicina Interna III, Ocular Hospital Universitario de San Carlos, Ciudad Universitaria, Madrid, Spain.
Abstract:
ANF binding sites were analysed in the ciliary processes of rabbits with unilateral experimental glaucoma which had been induced by injecting alpha-chymotrypsin into the posterior chamber of the right eyes. The intraocular pressure (IOP) of glaucomatous eyes was significantly greater (28.4 +/- 4 mmHg) than that of normotensive control eyes (13.1 +/- 1.4 mmHg, mean +/- S.E.M., n = 23, P less than 0.05). ANF concentrations in aqueous humour and the ciliary processes were significantly higher in glaucomatous eyes (91 +/- 2 pg ml-1 and 30.4 +/- 4.2 pg g-1 wet weight) than in normal eyes (3.1 +/- 2.2 pg ml-1 and 10.2 +/- 2.7 pg g-1 wet weight, respectively, n = 6, P less than 0.01). The number of ANF-binding sites (Bmax) in the ciliary processes of glaucomatous rabbit eyes was significantly decreased in comparison to the controls (24 +/- 4 vs. 13 +/- 3 fmol mg-1 protein, n = 10, P less than 0.05). These data suggest that ANF receptors in the ciliary processes are down-regulated and that ANF may play an important role in the pathophysiology of experimental glaucoma.