Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Facile Synthesis of Biocompatible Fluorescent Nanoparticles for Cellular Imaging and Targeted Detection of Cancer Cells.

ACS applied materials & interfaces·2015
Same author

Item exposure control for multidimensional computer adaptive testing under maximum likelihood and expected a posteriori estimation.

Behavior research methods·2015
Same author

[Electric-heat needling for 56 cases of cervical spondylosis with the neck type].

Zhongguo zhen jiu = Chinese acupuncture & moxibustion·2015
Same author

[Effects of GLP-1 Agonist Exenatide on Cardiac Diastolic Function and Vascular Endothelial Function in Diabetic Patients].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition·2015
Same author

Evaluation of The Cervista HPV A9 group In Screening Patients for Cervical Cancer.

Journal of medical screening·2015
Same author

Porous carbon derived from a metal-organic framework as an efficient adsorbent for the solid-phase extraction of phthalate esters.

Journal of separation science·2015
Same journal

[Advance in the application of molecular ruler-based RNA 3D dynamic analysis for the interpretation of variants of uncertain significance].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same journal

[Identification of a pregnant woman with tetragametic chimera of 46,XY/46,XX karyotype presenting as mixed agglutination during ABO blood group forward typing].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same journal

[Genetic analysis of a Chinese pedigree affected with Hereditary coagulation factor Ⅴ deficiency due to compound heterozygous variants IVS24+3A>T and p.Asp2222Gly].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same journal

[A novel TPM1 variant causing Dilated cardiomyopathy in a child: A case report and literature review].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same journal

[Clinical and genetic analysis of a child with Relapsing encephalopathy with cerebellar ataxia due to variant of ATP1A3 gene].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same journal

[Clinical and genetic analysis of a child with MRXS34 syndrome due to variant of NONO gene].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2026

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis
10:04

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Published on: May 1, 2015

[A modified cytogenetic study for multiple myeloma].

You-wen Qin1, Dao-lin Wei, Chun Wang

  • 1Department of Heamatology, First People's Hospital, Shanghai Jiaotong University, Shanghai, 200080 P.R.China.

Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics
|April 11, 2006
PubMed
Summary
This summary is machine-generated.

A modified 6-day culture method with cytokines significantly improves cytogenetic analysis for multiple myeloma (MM), revealing complex karyotypes in patients with poor chemotherapy response.

More Related Videos

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
07:53

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

Published on: April 15, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis
10:04

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Published on: May 1, 2015

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
07:53

Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma

Published on: April 15, 2022

Area of Science:

  • Hematology
  • Cytogenetics
  • Oncology

Context:

  • Multiple myeloma (MM) diagnosis and prognosis rely on accurate cytogenetic analysis.
  • Conventional cytogenetic methods often yield insufficient results for MM patients.
  • There is a need for improved techniques to detect chromosomal abnormalities in MM.

Purpose:

  • To evaluate the efficacy of a modified cell culture technique for cytogenetic analysis in MM.
  • To determine the clinical significance of chromosomal abnormalities detected through this enhanced method.

Summary:

  • Mononuclear cells from 20 MM patients were cultured for 3 days without cytokines and 6 days with IL-6 and GM-CSF, alongside direct analysis.
  • The 6-day culture with cytokines successfully identified karyotype abnormalities in 4 of 16 evaluable patients, including complex karyotypes.
  • Direct and 3-day cultures failed to detect abnormalities, while the 6-day method showed a correlation between abnormalities, advanced disease stage, and poor treatment response.

Impact:

  • Extended cytokine-supported culture enhances the efficiency of cytogenetic analysis for multiple myeloma.
  • Complex karyotypes are associated with advanced MM and resistance to chemotherapy, guiding treatment strategies.