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Updated: Aug 9, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
[Analysis of mitochondrial 12S rRNA gene polymorphism in cattle]
Yan Meng1, Shang-Zhong Xu, Lin-Sen Zan
1Animal Sciences and Technology College, Northwest A&F University, Shaanxi 712100, China. mengyanlhw@163.com
Abstract:
The mitochondrial DNA 12S rRNA in seven Chinese local yellow cattle breeds, one cultivated breed and three imported breeds was studied by single-strand conformation polymorphism (SSCP). Three haplotypes (I, II , III) were detected, with haplotype I found only in one cattle of the Jinnan breed. Haplotype II did not appear in the foreign cattle breeds and low in Chinese-Simmental breed but was rich in Chinese local breeds. Haplotype III had the widest distribution and was present in ten cattle breeds. The average polymorphism information content (PIC) for Chinese cattle between 0.232-0.423 was modest, which suggested relative abundance of genetic resources for the Chinese native cattle.
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