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Updated: Aug 9, 2026

09:46
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Expression of human LNalpha4LG4-5 in Pichia yeast]
Ji-Qin Lian1, Xu-Fang Dai, Xiao-Hui Li
1Faculty of Basic Medical Science, Third Military Medical University, Chongqing 400038, China.
Abstract:
To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.

