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Increased inositol phosphate accumulation in platelets from patients with NIDDM
H Ishii1, F Umeda, T Hashimoto
1Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Diabetes Research and Clinical Practice
|October 1, 1991
Summary
Diabetic patients show enhanced platelet activation due to increased inositol phosphate accumulation. This suggests a link between altered platelet signaling and accelerated functions in diabetes mellitus.
Area of Science:
- Biochemistry
- Hematology
- Endocrinology
Background:
- Platelet activation is crucial in hemostasis and thrombosis.
- Diabetes mellitus is associated with an increased risk of thrombotic events.
- Altered platelet signaling pathways may contribute to hypercoagulability in diabetes.
Purpose of the Study:
- To investigate thrombin-induced inositol phosphate accumulation in platelets from patients with non-insulin-dependent diabetes mellitus.
- To determine if altered inositol phosphate metabolism correlates with enhanced platelet aggregation in diabetes.
Main Methods:
- Platelets from diabetic patients and age-matched controls were labeled with [3H]inositol.
- Thrombin stimulation was used to induce inositol phosphate accumulation.
- Levels of inositol monophosphate, bisphosphate, and trisphosphate were measured.
- Platelet aggregation rates were assessed using aggregometry.
Main Results:
- No significant differences in basal [3H]inositol incorporation or phosphoinositide content were observed.
- Thrombin induced a dose- and time-dependent increase in inositol phosphate accumulation.
- Accumulation of [3H]inositol trisphosphate and [3H]inositol bisphosphate was significantly enhanced in diabetic platelets.
- Platelet aggregation rates induced by thrombin were also significantly enhanced in diabetic patients.
Conclusions:
- Increased inositol phosphate accumulation, particularly trisphosphate and bisphosphate, is a feature of platelets from patients with non-insulin-dependent diabetes mellitus.
- Enhanced inositol phosphate signaling correlates with accelerated platelet aggregation in diabetes.
- These findings suggest that altered platelet signaling contributes to hyperactive platelet function in diabetes mellitus, potentially increasing thrombotic risk.