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Updated: Aug 9, 2026
Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
Published on: September 15, 2023
Visualization, quantification and therapeutic evaluation of angiogenic vessels in cancer by synchrotron
T Sekka1, S A Volchikhina, A Tanaka
1Department of Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Abstract:
The usefulness of a synchrotron microangiography system for depicting, quantitating and therapeutically evaluating angiogenic vessels in cancer is illustrated. In 20 mice transplanted with murine colon cancer, sequential changes in the angiogenic vessels were determined by using synchrotron microangiography, using changes in tumor volume for reference. This system allowed the depiction and quantification of angiogenic vessels in the period from one to four weeks after transplantation. The effects of antiangiogenic therapy were evaluated by using a neutralizing antibody against vascular endothelial growth factor. The neutralizing antibody partially suppressed angiogenesis and tumor growth. Synchrotron microangiography is shown to be useful for the depiction, quantification and evaluation of angiogenic vessels in cancer.
Insights
Synchrotron microangiography effectively visualizes and quantifies cancer
Area of Science:
- Biomedical imaging
- Cancer research
- Angiogenesis studies
Background:
- Angiogenesis, the formation of new blood vessels, is crucial for tumor growth and metastasis.
- Accurate depiction and quantification of angiogenic vessels are vital for cancer research and therapeutic evaluation.
Purpose of the Study:
- To illustrate the utility of synchrotron microangiography for visualizing, quantifying, and evaluating angiogenic vessels in cancer.
- To assess the impact of antiangiogenic therapy on tumor angiogenesis and growth.
Main Methods:
- Utilized synchrotron microangiography to monitor sequential changes in angiogenic vessels in 20 mice with murine colon cancer.
- Correlated vessel changes with tumor volume over a four-week period.
- Evaluated antiangiogenic therapy using a neutralizing antibody against vascular endothelial growth factor (VEGF).
Main Results:
- Synchrotron microangiography successfully depicted and quantified angiogenic vessels from one to four weeks post-transplantation.
- The neutralizing antibody against VEGF partially suppressed angiogenesis and tumor growth.
Conclusions:
- Synchrotron microangiography is a valuable tool for the depiction, quantification, and therapeutic evaluation of tumor angiogenesis.
- This imaging modality aids in understanding antiangiogenic treatment effects in preclinical cancer models.
