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Updated: Aug 1, 2026

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
Published on: June 26, 2019
[Effects of bFGF and alpha-MSH on adhesion and migration of human melanocytes in vitro]
Xian-qi Zhang1, Jie Feng, Kuan-hou Mou
1Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China. zhangxianqi68@163.com
Objective:
To observe the effect of basic fibroblast growth factor (bFGF) and alpha-melanocyte stimulating hormone (alpha-MSH) on adhesion and migration of melanocytes in vitro.
Methods:
Human melanocytes were obtained from normal human foreskins. Culture dishes covered with fibronectin were used to perform melanocytes adhesion assay, and cell motility was assessed using the Transwell micropore filter method.
Result:
bFGF and alpha-MSH increased melanocytes adhesion on culture dishes covered with fibronectin. bFGF stimulated melanocytes migration through micropore filter while alpha-MSH had no significant effects.
Conclusion:
bFGF and alpha-MSH could promote the adhesion and migration of melanocytes, which suggests that two agents may play a role in the repigmentation of vitiligo.
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