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Updated: Aug 9, 2026

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Published on: June 30, 2020
[Separation of vitamins by non-conventional techniques. Facilitated pertraction of vitamin C]
Anca-Irina Galaction1, Alexandra-Cristina Blaga, D Caşcaval
1Catedra de Substanţe Bioactive si Biotehnologii Medicale, Facultatea de Bioinginerie Medicală, Universitatea de Medicină si Farmacie "Gr. T. Popa", Iaşi.
Abstract:
Pertraction (permeation through liquid membranes) is one of the new techniques applied for separation and advanced purification of bioactive compounds. The study on the facilitated pertraction of vitamin C with Amberlite LA-2 underlined the significant influences of the pH-gradient between the aqueous phases, carrier concentration and mixing intensity. Thus, for reaching the maximum efficiency of vitamin C pertraction, the pH-value of the initial aqueous phase below 2, the pH-value of the final aqueous solution greater than 11 and the Amberlite LA-2 concentration in solvent layer over 80 g/l are required.
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