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Linezolid and human polymorphonuclear leukocyte function
Are Naess1, Kristin Stenhaug Kilhus, Tone W Nystad
1Institute of Medicine, University of Bergen, Norway. are.nass@med.uib.no
Chemotherapy
|April 14, 2006
Summary
Linezolid, an oxazolidinone antibiotic, does not affect human polymorphonuclear leukocyte (PMN) function, including chemotaxis, phagocytosis, and respiratory burst. This finding holds true for both pure and commercial preparations at therapeutic concentrations.
Area of Science:
- Immunology
- Pharmacology
- Microbiology
Background:
- Linezolid is a novel oxazolidinone antibiotic.
- Human polymorphonuclear leukocytes (PMNs) are critical immune cells.
Purpose of the Study:
- To investigate the impact of linezolid on human PMN function.
- To assess linezolid's effects on key immune cell activities.
Main Methods:
- Flow cytometry was employed to analyze PMN chemotaxis.
- Phagocytosis and respiratory burst assays were conducted after linezolid incubation.
Main Results:
- Linezolid, at concentrations ranging from 10-160 mg/l, showed no significant effect on PMN chemotaxis.
- Phagocytosis and respiratory burst activities of PMNs remained unaffected by linezolid exposure.
Conclusions:
- Linezolid does not influence human PMN function at therapeutic or supratherapeutic concentrations.
- This effect is consistent for both the pure form of linezolid and its commercial preparation for intravenous use.