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Evaluation of chemopreventive action of Ginsenoside Rp1
Ashok Kumar1, Madhu Kumar, Meenakshi Panwar
1Radiation and Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur-302004, India. mamsjpr@hotmail.com
Abstract:
We evaluated the chemopreventive properties of Ginsenoside Rp1 on 7,12-Dimethyl benz (a) anthracene (DMBA) skin papillomagenesis in Swiss albino mice. A significant reduction in values of tumor incidence, tumor burden, and cumulative number of papilloma was observed in mice treated orally with Ginsenoside Rp1 continuously at pre-, peri- and post-initiational stages of papillomagenesis as compared to the control group. Chemopreventive potential of Ginsenoside Rp1 was also observed on the skin metabolizing enzymes in Swiss albino mice. Ginsenoside Rp1 produced a significant elevation in the skin microsomal cytochrome p-450 and cytochrome b5, glutathione S-transferase (GST), reduced glutathione (GSH), glutathione peroxidase (GPX), glutathione reductase (GR), DT-diaphorase, superoxide dismutase (SOD) and catalase levels in the group of mice treated with Ginsenoside Rp1 for seven consecutive days. However, there was significant decrease in lipid peroxidation (LPO) level in Ginsenoside Rp1 treated group.
Insights
Ginsenoside Rp1 demonstrated chemopreventive properties against skin papillomagenesis in mice. It reduced tumor development and modulated skin metabolizing enzymes, suggesting a protective role.
Area of Science:
- Pharmacology
- Oncology
- Biochemistry
Background:
- Skin cancer remains a significant global health concern.
- Chemoprevention offers a promising strategy for cancer prevention.
- Ginsenosides, derived from Panax ginseng, are known for their diverse biological activities.
Purpose of the Study:
- To investigate the chemopreventive efficacy of Ginsenoside Rp1 against 7,12-Dimethyl benz (a) anthracene (DMBA)-induced skin papillomagenesis in Swiss albino mice.
- To evaluate the effect of Ginsenoside Rp1 on skin metabolizing enzymes during papillomagenesis.
Main Methods:
- Swiss albino mice were subjected to DMBA skin application to induce papillomagenesis.
- Ginsenoside Rp1 was administered orally at pre-, peri-, and post-initiational stages.
- Levels of various skin metabolizing enzymes, including cytochrome P450, GST, GSH, GPX, GR, DT-diaphorase, SOD, catalase, and lipid peroxidation (LPO), were assessed.
Main Results:
- Ginsenoside Rp1 treatment significantly reduced tumor incidence, tumor burden, and the cumulative number of papillomas.
- Oral administration of Ginsenoside Rp1 led to a significant elevation in the activity of skin microsomal enzymes: cytochrome P450, cytochrome b5, glutathione S-transferase (GST), reduced glutathione (GSH), glutathione peroxidase (GPX), glutathione reductase (GR), DT-diaphorase, superoxide dismutase (SOD), and catalase.
- A significant decrease in lipid peroxidation (LPO) levels was observed in the Ginsenoside Rp1-treated group.
Conclusions:
- Ginsenoside Rp1 exhibits significant chemopreventive properties against DMBA-induced skin papillomagenesis in mice.
- The protective effects of Ginsenoside Rp1 are associated with the modulation of key skin metabolizing enzymes and antioxidant defense systems.
- Ginsenoside Rp1 holds potential as a therapeutic agent for skin cancer prevention.