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Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
Published on: May 17, 2019
Expression of interleukin 11 and its receptor and their prognostic value in human breast cancer
Satheesha Hanavadi1, Tracey A Martin, Gareth Watkins
1University Department of Surgery, Wales College of Medicine, Cardiff University, Heath Park, Cardiff, CF4 4XN, United Kingdom. satheeshh@yahoo.com
Background:
Recent experimental evidence has shown a potential role of interleukin (IL)-11 and its receptor in breast cancer development and progression. However, there is little clinical information to support this hypothesis. We examined the expression of IL-11 and its receptor in primary breast cancer tissue samples and correlated their level of expression with the clinical outcome.
Methods:
Primary breast cancer samples (n=109) and matched background tissue obtained from patients in the cohort (n=33) were processed for frozen section and RNA extraction. Frozen sections from matched tissues were immunostained with IL-11 and IL-11 receptor antibodies. Staining intensity was analyzed by computer image analysis. RNA was reverse-transcribed and quantified before analysis by quantitative polymerase chain reaction. Results were expressed as the number of transcripts (standardized by beta-actin). The data were compared with the clinical outcome of the disease.
Results:
The intensity of staining for both IL-11 and the IL-11 receptor was distinctly high in tumor samples (P<.01). The transcript level of IL-11 was significantly higher in node-positive tumor samples compared with node-negative samples (P=.02). Tumors with a poor prognostic index and poor histological grade showed a higher level of IL-11. A higher level of IL-11 was linked to poorer survival with Kaplan-Meier survival analysis.
Conclusions:
IL-11 can be a predictor of poor prognosis in human breast cancer.