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Updated: Jul 31, 2026

Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
Effect of brassinolide on tyrosine phosphorylation of pea leaf proteins
E O Fedina1, F G Karimova, I A Tarchevsky
1Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, 420111 Kazan, Russia. fedina@mail.knc.ru
Abstract:
Brassinosteroid-induced phosphorylation of tyrosine residues in proteins was studied. Proteins of crude extract of pea leaves were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed 7 and 13 tyrosine-phosphorylated proteins, respectively. Brassinolide increased the phosphorylation level of most of these proteins. With inhibitors of tyrosine protein phosphatases, such as phenylarsine oxide and orthovanadate, the level of tyrosine phosphorylation of these proteins increased.
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