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Updated: Aug 9, 2026
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
Published on: December 26, 2019
Effects of interferon-gamma reverse hypoxia-stimulated extracellular matrix expression in human peritoneal and
Ghassan M Saed1, Michael P Diamond
1Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University School of Medicine-Detroit Medical Center, Detroit, Michigan, USA. g.saed@wayne.edu
Objective:
To determine the response of adhesion and normal peritoneal fibroblasts to interferon-gamma (IFN-gamma) under normal and hypoxic conditions.
Design:
Prospective experimental study.
Setting:
University medical center.
Patient(S):
Primary cultures of fibroblasts established from peritoneal and adhesion tissue of the same patients.
Intervention(S):
Hypoxia and IFN-gamma treatment of the primary cultured fibroblasts.
Main Outcome Measure(S):
Primary cultures of fibroblasts were established from peritoneal and adhesion tissues of the same patients (n = 5). The expression of extracellular matrix components (type I collagen and fibronectin) in adhesion and peritoneal fibroblasts under normal (20% O2) and hypoxic (2% O2) conditions was evaluated by multiplex reverse-transcription polymerase chain reaction analysis.
Result(S):
Adhesion fibroblasts (ADF) have increased basal levels of type I collagen as compared with normal peritoneal fibroblasts (NF). Interferon-gamma treatment resulted in a dose-response decrease in type I collagen and fibronectin mRNA levels in both ADF and NF. Hypoxia treatment resulted in a time-response increase in type I collagen and fibronectin mRNA levels in NF and ADF. Hypoxia had no effect on type I collagen and fibronectin mRNA levels in the presence of increasing dose of IFN-gamma in both NF and ADF. Interferon-gamma can block the stimulating effects of hypoxia on type I collagen expression, supporting the antifibrogenic nature of this cytokine.
Conclusion(S):
Understanding the mechanism by which IFN-gamma exerts its effect will be important in the utilization of this cytokine as a therapy for postoperative adhesion and tissue fibrosis.
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