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Effect of solid storage on caprine semen conservation at 5 degrees C.
I Salvador1, J Yániz, M P Viudes-de-Castro
1Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Moncada, Valencia, Spain.
Theriogenology
|April 18, 2006
Summary
Solid media extenders improved buck semen motility during 5°C storage. Washing semen also enhanced sperm viability, suggesting solid storage and washing are effective for goat semen preservation.
Area of Science:
- Reproductive Biology
- Animal Science
- Semen Preservation
Background:
- Optimizing buck semen preservation is crucial for artificial insemination programs.
- Current extenders may not fully maintain sperm viability during refrigerated storage.
- Investigating novel storage methods like solid-phase extenders is needed.
Purpose of the Study:
- To evaluate the efficacy of solid-phase extenders for buck semen storage at 5°C.
- To determine the impact of cysteine supplementation and seminal plasma washing on sperm survival.
- To compare solid-phase extenders with traditional milk-based extenders in a field trial.
Main Methods:
- In vitro assessment of sperm motility, acrosome integrity (NAR), and hypo-osmotic swelling test (HOST).
- Comparison of milk-based (M), solid-phase (G), cysteine-supplemented (C), and combined (GC) extenders.
- Artificial insemination field trial comparing solid-phase (G) and milk-based (M) extenders.
Main Results:
- Solid-phase extenders (G) significantly maintained higher sperm motility compared to other extenders.
- Washing seminal plasma significantly improved motility, NAR, and HOST.
- Cysteine supplementation did not enhance sperm viability; no significant fertility differences between G and M extenders.
Conclusions:
- Solid-phase extenders, particularly when combined with semen washing, offer a successful method for preserving buck semen at 5°C.
- Washing of buck semen is beneficial for improving sperm quality parameters during storage.
- Further research may optimize solid-phase extender composition for enhanced fertility outcomes.