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Related Experiment Videos

A buffering SERCA pump in models of calcium dynamics.

Erin R Higgins1, Mark B Cannell, James Sneyd

  • 1Department of Mathematics, School of Medical Sciences, The University of Auckland, Auckland, New Zealand.

Biophysical Journal
|April 18, 2006
PubMed
Summary

This study models the buffering sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) pump. Buffering impacts calcium oscillation amplitude and period, and enables adaptation and reduced futile cycling.

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Area of Science:

  • Cellular Biology
  • Biophysics
  • Biochemistry

Background:

  • Cellular communication relies on calcium oscillations.
  • Calcium influx from the endoplasmic reticulum (ER) and efflux back into the ER drive these oscillations.
  • The sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) pump regulates calcium transport between the cytosol and ER.

Purpose of the Study:

  • To model a buffering SERCA pump.
  • To investigate the effects of SERCA pump buffering on calcium oscillation dynamics.
  • To explore SERCA pump adaptation and its role in preventing futile calcium cycling.

Main Methods:

  • Development of a computational model for a buffering SERCA pump.
  • Simulation of calcium oscillations using both buffering and non-buffering SERCA pump models.

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  • Analysis of oscillation amplitude, period, and adaptation responses.

    • Main Results:

    • Non-buffering SERCA pumps produced oscillations with larger amplitude and shorter period compared to buffering pumps.
    • The buffering SERCA pump model demonstrated adaptation to cellular stimuli.
    • A bidirectional SERCA pump model effectively eliminated futile calcium cycling at rest.

    Conclusions:

    • SERCA pump buffering significantly influences calcium oscillation characteristics.
    • SERCA pump buffering contributes to cellular adaptation mechanisms.
    • Bidirectional SERCA pump function is crucial for preventing energy-wasting calcium cycling.