CB1 cannabinoid receptor-mediated changes of trabecular meshwork cellular properties.

Area of Science:

• Ophthalmology
• Cell Biology
• Pharmacology

Background:

• Trabecular meshwork (TM) cells are crucial for regulating intraocular pressure.
• Cannabinoid receptors, particularly CB1, are implicated in various cellular processes.

Purpose of the Study:

• To investigate the role of CB1 cannabinoid receptors in TM cell functions.
• To assess the impact of CB1 receptor modulation on TM cell migration, adhesion, morphology, and cytoskeleton.

Main Methods:

• Cultured porcine TM cells were treated with a CB1 agonist (noladin ether) and antagonist (SR141716A).
• Cell migration was assessed using fluorescence-assisted transmigration invasion and motility assays (FATIMA) and wound healing assays.
• Cell adhesion was measured on fibronectin-coated plates.
• Morphological changes and cytoskeleton (actin filaments, focal adhesions) were examined using phalloidin staining and immunocytochemistry.

Main Results:

• Noladin ether inhibited TM cell migration in a concentration-dependent manner, an effect reversed by SR141716A.
• Noladin ether delayed wound healing and decreased TM cell adhesion to fibronectin, with these effects antagonized by SR141716A.
• Morphological analysis revealed cell rounding, reduced actin stress fibers, and decreased focal adhesions upon noladin ether treatment.

Conclusions:

• CB1 receptor activation by noladin ether significantly modulates TM cell migration, adhesion, and morphology.
• The observed cellular effects are mediated through CB1 cannabinoid receptors in TM cells.
• These findings highlight a potential role for CB1 receptors in regulating TM cell behavior.