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Updated: Aug 9, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[Isolation and fermentation culture of fungi from Cordyceps soofifera]
Dong-qing Cheng1, Zhi-shan Ding, Mei-ai Lin
1Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Objective:
To study the fungi isolated from Cordyceps sobolifera and its fermentation culture.
Methods:
The fungi was isolated and identified by its hypha and spores. Three liquid media were used in the culture.
Results:
Pure culture was gained and the fungi was identified to be Paecilomyces cicadae. The fungi can grow best in liquid media: egg 110 g + silkworm powder 30 g + VB1 2 tahlets + MgSO4 x 7H2O 0.5 g + K2HPO4 1 g + H2O 1000 ml, in which every litre can produce 135 g wet hypha after cultured 4 d.
Conclusion:
Paecilomyces cicadae from Cordyceps sobolifera can be cultured in liquid media.
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