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Updated: Aug 9, 2026
Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
Published on: June 22, 2012
One-step preparation of chitosan-coated cationic liposomes by an improved supercritical reverse-phase evaporation
Katsuto Otake1, Takeshi Shimomura, Toshihiro Goto
1Nanotechnology Research Institute and Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan. katsuto-otake@aist.go.jp
Abstract:
High-pressure carbon dioxide in contact with water dissolves to form carbonic acid, causing a decrease in pH. By use of these characteristics of a CO2/H2O biphasic system, chitosan-coated cationic liposomes of l-alpha-dipalmitoylphosphatidylcholine were successfully prepared by an improved supercritical reverse-phase evaporation (ISCRPE) method. Liposome-chitosan complexes carrying a positive charge were prepared in a single-step procedure without the use of acid or organic solvent, including ethanol. The maximum trapping efficiency of liposomes prepared by the ISCRPE method was 17%, with or without the addition of chitosan, compared to only 2% for liposomes prepared by the Bangham method. Furthermore, the liposomal dispersion was stable at room temperature in a sealed tube for over 30 days.
