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Comparison of 'real-time' and immunometric RT-PCR: RNA interference of reverse transcriptase-PCR

Margaret E Hoadley1, Stephen J Hopkins

  • 1Injury Research, University of Manchester Division of Medicine and Neurosciences, Clinical Sciences Building, Hope Hospital, Salford, M6 8HD, UK. mhoadley@fsl.ho.man.ac.uk

Journal of Immunological Methods
|April 19, 2006
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...