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Histomorphometric evaluation of bone grafts harvested by different methods
M Berengo1, C Bacci, M Sartori
1School of Dentistry, Department of Surgical Sciences, University of Padua, Padua, Italy.
Minerva Stomatologica
|April 19, 2006
Summary
Evaluating nine methods for harvesting autologous bone, this study found that rongeur pliers and en bloc harvesting yielded the best results for grafting. Burs and scrapers produced non-vital bone unsuitable for oral defect repair.
Area of Science:
- Oral surgery
- Bone grafting
- Histomorphometry
Background:
- Autologous bone is frequently used for oral bony defects.
- Numerous protocols exist for harvesting particulate bone.
- Qualitative evaluation of harvested bone quality is lacking.
Purpose of the Study:
- To qualitatively evaluate autologous bone harvested using nine different methods.
- To compare the viability and osteocyte presence in bone harvested by various techniques.
Main Methods:
- Nine harvesting techniques were applied to retromolar bone.
- Microphotography and histomorphometric analysis were used.
- Evaluated parameters included particle size, vital bone percentage, and osteocyte count.
Main Results:
- Burs and safe scraper yielded 100% non-vital bone with no osteocytes.
- Intermediate non-vital bone percentages were observed with back-action chisel, gouge chisel, spiral bur, and piezosurgery.
- Rongeur pliers and en bloc harvesting provided the best results.
Conclusions:
- Rongeur pliers and en bloc harvesting are recommended for obtaining vital autologous bone.
- Gouge shaped bone chisel, back-action chisel, and piezosurgery are viable alternatives, though piezosurgery may leave gaps.
- Methods using burs or safe scrapers are unsuitable for grafting due to non-vital bone content.