Related Experiment Video
Updated: Aug 3, 2026
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Published on: April 6, 2012
Myc target transcriptomes
1Department of Medicine, The Johns Hopkins University School of Medicine, Ross 1032, 720 Rutland Avenue, Baltimore, MD 21205, USA. llee12@jhmi.edu
Abstract:
The c-Myc oncogenic transcription factor plays a central role in many human cancers through the regulation of gene expression. Although the molecular mechanisms by which c-Myc and its obligate partner, Max, regulate gene expression are becoming better defined, genes or transcriptomes that c-Myc regulate are just emerging from a variety of different experimental approaches. Studies of individual c-Myc target genes and their functional implications are now complemented by large surveys of c-Myc target genes through the use of subtraction cloning, DNA microarray analysis, serial analysis of gene expression (SAGE), chromatin immunoprecipitation, and genome marking methods. To fully appreciate the differences between physiological c-Myc function in normal cells and deregulated c-Myc function in tumors, the challenge now is to determine how the authenticated transcriptomes effect the various phenotypes induced by c-Myc and to define how c-Myc transcriptomes are altered by the Mad family of proteins.
Insights
The c-Myc oncogenic transcription factor regulates gene expression in human cancers. New methods are identifying c-Myc target genes to understand its role in normal cells versus tumors.
Area of Science:
- Molecular biology
- Oncology
- Gene regulation
Background:
- The c-Myc oncogenic transcription factor is crucial in human cancer development.
- Understanding c-Myc's role in gene regulation is key to cancer research.
- Its obligate partner, Max, works with c-Myc to control gene expression.
Purpose of the Study:
- To identify and characterize the full spectrum of genes regulated by c-Myc (c-Myc target genes).
- To differentiate the functions of c-Myc in normal physiological states versus cancerous conditions.
- To investigate how the Mad protein family influences c-Myc-regulated transcriptomes.
Main Methods:
- Utilizing various experimental approaches including subtraction cloning and DNA microarray analysis.
- Employing high-throughput techniques such as serial analysis of gene expression (SAGE).
- Applying chromatin immunoprecipitation and genome marking methods to map c-Myc targets.
Main Results:
- Emerging data is revealing extensive lists of c-Myc regulated genes and transcriptomes.
- Studies are beginning to link these transcriptomes to specific cellular phenotypes induced by c-Myc.
- Differences in c-Myc function between normal and tumor cells are becoming clearer.
Conclusions:
- Comprehensive identification of c-Myc target genes is essential for understanding its oncogenic potential.
- Further research is needed to connect c-Myc transcriptomes to cancer phenotypes.
- The influence of Mad proteins on c-Myc regulation warrants further investigation.
More Related Videos
Related Concept Videos
02:23Master Transcription Regulators
02:23Master Transcription Regulators