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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Electrophoresis: Overview01:20

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

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Related Experiment Video

Updated: Jul 23, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Urine profiling using capillary electrophoresis-mass spectrometry and multivariate data analysis.

Sara Ullsten1, Rolf Danielsson, Daniel Bäckström

  • 1Department of Analytical Chemistry, Biomedical Centre, Uppsala University, P.O. Box 599, SE-751 24 Uppsala, Sweden.

Journal of Chromatography. A
|April 20, 2006
PubMed
Summary

This study developed a fast capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESIMS) method for urine metabolic profiling. The technique successfully distinguished between blank and paracetamol-dosed human urine samples using multivariate data analysis.

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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

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Last Updated: Jul 23, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Area of Science:

  • Analytical Chemistry
  • Metabolomics
  • Biochemistry

Background:

  • Metabolic profiling of biological samples like urine is crucial for understanding physiological and pathological states.
  • Existing methods may lack speed, generality, or the ability to analyze complex mixtures without prior compound knowledge.

Purpose of the Study:

  • To develop a rapid and general method for comprehensive urine metabolic profiling.
  • To demonstrate the utility of the developed method in distinguishing between pre- and post-drug intake samples.
  • To identify key metabolites responsible for observed sample differentiation.

Main Methods:

  • Capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESIMS) was employed for sample separation and detection.
  • Human urine samples were analyzed under both acidic and basic conditions, using positive and negative ion modes.
  • Multivariate data analysis, including pair-wise 'fuzzy' correlation and eigenvalue analysis, was applied to the complex datasets.

Main Results:

  • The developed CE-ESIMS method provided fast and efficient metabolic profiling of urine.
  • Multivariate analysis successfully discriminated between blank urine and urine collected after paracetamol administration.
  • Two-dimensional loadings plots aided in identifying specific m/z values of differentiating compounds.

Conclusions:

  • The presented method offers a general and rapid approach for urine metabolic profiling.
  • This technique, coupled with multivariate data analysis, is effective for biomarker discovery and sample classification.
  • The approach facilitates the identification of metabolites indicative of physiological changes, such as drug metabolism.