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Related Experiment Video

Updated: Jan 10, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
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DNA immobilisation procedures for surface plasmon resonance imaging (SPRI) based microarray systems.

Ilaria Mannelli1, Laure Lecerf, Mohamed Guerrouache

  • 1Laboratoire Charles Fabry de l'Institut d'Optique, Centre National de la Recherche Scientifique CNRS UMR 8501, Bât 503, Université Paris Sud-XI, 91403 Orsay, France.

Biosensors & Bioelectronics
|April 20, 2006
PubMed
Summary
This summary is machine-generated.

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This study developed two surface chemistries for DNA microarray affinity sensors using surface plasmon resonance imaging. Both methods successfully detected cystic fibrosis mutations, demonstrating their potential for genetic disease diagnosis.

Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Surface Chemistry

Background:

  • Development of sensitive and specific DNA microarray affinity sensors is crucial for genetic disease diagnosis.
  • Surface plasmon resonance imaging (SPRI) offers a label-free, real-time detection method for biomolecular interactions.
  • Evaluating different surface chemistries is essential for optimizing sensor performance.

Purpose of the Study:

  • To develop and compare two distinct surface chemistries for SPRI-based DNA microarray affinity sensors.
  • To assess the capability of these sensors in detecting gene mutations associated with human diseases, specifically cystic fibrosis.
  • To evaluate the real-time, parallel detection of unlabeled oligonucleotide targets against immobilized probes.

Main Methods:

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  • Fabrication of two surface chemistries: 11-mercaptoundecanoic acid-poly(ethylenimine) (MUA-PEI) using electrostatic interactions and a dextran procedure using covalent bonds.
  • Immobilization of oligonucleotide probes in a 14x14 matrix (196 spots) on functionalized sensor surfaces.
  • Real-time monitoring of hybridization between solution-phase target sequences and immobilized probes using SPRI.

    • Main Results:

    • Both MUA-PEI and dextran surfaces demonstrated successful immobilization of oligonucleotide probes.
    • The SPRI system effectively monitored hybridization in real-time and in parallel.
    • The sensors accurately detected multiple cystic fibrosis mutations (DeltaF508, DeltaI507, M470V, Q493X, V520F, 1716 G>A) within the CFTR gene.
    • Both surface chemistries exhibited the ability to discriminate between normal and mutant sequences differing by a single nucleotide polymorphism.

    Conclusions:

    • The developed SPRI-based DNA microarray affinity sensors with MUA-PEI and dextran surface chemistries are effective for genetic mutation detection.
    • These sensors show significant potential for the parallel diagnosis of genetic diseases like cystic fibrosis.
    • The ability to detect single nucleotide differences highlights the high specificity and sensitivity of the developed platform.