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Related Experiment Videos

Accessory gene regulator locus of Staphylococcus intermedius.

Julia M L Sung1, Peter D Chantler, David H Lloyd

  • 1Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, United Kingdom.

Infection and Immunity
|April 20, 2006
PubMed
Summary

The accessory gene regulator (agr) gene in Staphylococcus intermedius was fully sequenced, revealing genetic variations and at least three agr specificity groups. This study elucidates agr gene regulation by self-secreted peptides.

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Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Molecular Biology

Background:

  • The accessory gene regulator (agr) locus is crucial for regulating virulence factor production in Staphylococcus intermedius.
  • Characterization of the agr gene is essential for understanding pathogenicity and developing targeted interventions.

Purpose of the Study:

  • To obtain the complete sequence of the agr gene in Staphylococcus intermedius.
  • To investigate genetic variations within the agr gene, specifically in the autoinducing peptide (AIP).
  • To elucidate the regulatory mechanism of the agr system and its effector molecule, RNAIII.

Main Methods:

  • PCR-based genome walking for complete agr gene sequencing.
  • DNA sequencing of agrD PCR products from 20 isolates.

Related Experiment Videos

  • Phylogenetic analysis for agr gene classification.
  • Real-time PCR to study RNAIII expression.
  • Synthesis and testing of novel cyclic peptides.

    • Main Results:

    • The first complete 3,436 bp sequence of the Staphylococcus intermedius agr gene was obtained, identifying five open reading frames (ORFs).
    • Sequence analysis revealed at least three distinct agr specificity groups within S. intermedius based on agrD variations.
    • RNAIII, the agr effector molecule, is regulated in an autocrine manner and correlates with luk and entC gene expression.
    • Novel cyclic peptides, lacking cysteine, were synthesized and shown to up-regulate RNAIII, supporting autocrine activation of the agr gene.

    Conclusions:

    • The study provides a comprehensive characterization of the Staphylococcus intermedius agr gene and its regulatory elements.
    • Genetic diversity in the agrD gene defines at least three specificity groups, impacting virulence factor regulation.
    • Autocrine signaling via novel cyclic peptides plays a key role in activating the agr system in S. intermedius.