Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Carbohydrate recognition by Clostridium difficile toxin A.

Antonio Greco1, Jason G S Ho, Shuang-Jun Lin

  • 1Alberta Ingenuity Centre for Carbohydrate Science, University of Calgary, Calgary, Canada T2N 1N4.

Nature Structural & Molecular Biology
|April 20, 2006
PubMed
Summary

Clostridium difficile TcdA toxin binds intestinal cells via specific carbohydrate interactions. A structural study reveals how trisaccharides bind TcdA, offering insights for developing new therapeutics targeting this toxin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment of in-stent restenosis with ultrathin-strut versus thin-strut drug-eluting stents or drug-eluting balloons: a multicentre registry.

EuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology·2024
Same author

Sudden Visual Loss After Vitreoretinal Surgery: A Case Report.

Case reports in ophthalmological medicine·2024
Same author

Differentiation of Benign and Malignant Neck Neoplastic Lesions Using Diffusion-Weighted Magnetic Resonance Imaging.

Journal of imaging·2024
Same author

Downstream cangrelor versus upstream ticagrelor in patients with ST-segment elevation myocardial infarction: A propensity score-matched analysis.

International journal of cardiology·2024
Same author

Non-invasive physiological assessment of intermediate coronary stenoses from plain angiography through artificial intelligence: the STARFLOW system.

European heart journal. Quality of care & clinical outcomes·2024
Same author

Response by Giacoppo et al to Letter Regarding Article "Coronary Angiography, Intravascular Ultrasound, and Optical Coherence Tomography for Guiding of Percutaneous Coronary Intervention: A Systematic Review and Network Meta-Analysis".

Circulation·2024

Area of Science:

  • Microbiology and Toxinology
  • Structural Biology
  • Carbohydrate Chemistry

Background:

  • Clostridium difficile toxin A (TcdA) is a key virulence factor responsible for C. difficile infection.
  • TcdA mediates its effects by binding to intestinal epithelial cells, a process involving carbohydrate recognition.

Purpose of the Study:

  • To elucidate the structural basis of TcdA binding to specific carbohydrate moieties.
  • To identify conserved binding sites and interactions for potential therapeutic targeting.

Main Methods:

  • Cocrystallography at 2-Å resolution was employed to determine the structure of TcdA bound to a trisaccharide.
  • Analysis of conserved residues across multiple putative binding sites.

Main Results:

Related Experiment Videos

  • The study determined the high-resolution cocrystal structure of TcdA with two molecules of a specific trisaccharide (alpha-Gal-(1,3)-beta-Gal-(1,4)-beta-GlcNAcO(CH(2))(8)CO(2)CH(3)).
  • The trisaccharides were observed to bind in an extended conformation.
  • Key interacting residues with the trisaccharides are conserved across seven identified binding sites on TcdA.

Conclusions:

  • The conserved residues and multivalent binding mode suggest a specific mechanism for TcdA carbohydrate recognition.
  • This structural understanding provides a foundation for the rational design of novel therapeutics aimed at inhibiting TcdA binding and activity.