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[Promotion of intestinal drug absorption by milk fat globule membrane]
H X Liu1, I Adachi, I Horikoshi
1Department of Hospital Pharmacy, Toyama Medical and Pharmaceutical University, Japan.
Abstract:
A soybean oil emulsion was prepared by using milk fat globule membrane (MFGM) materials as an emulsifier. Its stability and effect on the intestinal absorption of vitamin D3, amphotericin B and kanamycin were studied. The MFGM emulsion was as highly stable as a Tween 80 emulsion. When the MFGM emulsion was sonicated with ultrasonic disrupter, the emulsion became more stable. Quantification of vitamin D3 in the intestinal lymph indicated that the MFGM emulsion enhanced lymphatic absorption of vitamin D3. The recovered percentage of vitamin D3 emulsified with MFGM in the lymph was 1.4 times that by Tween 80, and 2.5 times that of oil-in-water suspension. Statistically significant difference was found among them (p less than 0.05). The volumes of an oil phase in the emulsion affected the absorption of vitamin D3. The recovered percentage of vitamin D3 from the lymph increased with reducing the volume of the oil phase. Sonication of the emulsion did not affect the intestinal drug absorption. Furthermore, the MFGM emulsion had no effect on the intestinal absorption of amphotericin B and kanamycin.
Insights
Milk fat globule membrane (MFGM) creates a stable soybean oil emulsion that significantly enhances intestinal vitamin D3 absorption. This novel emulsion did not affect the absorption of amphotericin B or kanamycin.
Area of Science:
- Food science and technology
- Biochemistry
- Pharmacology
Context:
- Developing effective delivery systems for lipophilic compounds like vitamin D3 is crucial for enhancing bioavailability.
- Traditional emulsifiers like Tween 80 have limitations.
- Milk fat globule membrane (MFGM) presents a natural and potentially superior alternative for emulsion formulation.
Purpose:
- To formulate a stable soybean oil emulsion using MFGM as an emulsifier.
- To investigate the impact of MFGM emulsion on the intestinal absorption of vitamin D3, amphotericin B, and kanamycin.
- To compare the efficacy of MFGM emulsion with Tween 80 emulsion and oil-in-water suspension for vitamin D3 absorption.
Summary:
- A stable soybean oil-in-water emulsion was successfully prepared using MFGM materials.
- The MFGM emulsion demonstrated enhanced lymphatic absorption of vitamin D3, with recovery rates significantly higher than Tween 80 or oil-in-water suspension.
- Emulsion stability was further improved by sonication, though this did not impact drug absorption. MFGM emulsion did not affect the absorption of amphotericin B and kanamycin.
Impact:
- MFGM-based emulsions offer a promising approach for improving the oral bioavailability of lipophilic vitamins.
- This research provides a foundation for developing novel food or pharmaceutical delivery systems utilizing natural emulsifiers.
- The findings suggest MFGM emulsions can be tailored to optimize the absorption of specific nutrients without interfering with other drug absorptions.