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Updated: Aug 9, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
[Human subsistence strategy in Neolithic times: an isotopic analysis of Bavarian skeletal findings]
Tanja Asam1, Gisela Grupe, Joris Peters
1Department für Biologie 1, Biodiversitätsforschung/Anthropologie, Biozentrum der Ludwig-Maximilians-Universität München, Martinsried.
Abstract:
Originating from the Near East, the Neolithic lifestyle will reach Southeast Europe in its fully developed form in the course of the 7th millennium cal. BC. In the region of today's Bavaria this lifestyle can be evidenced from the middle of the 6th millennium cal. BC onwards. Stable isotope analyses of carbon and nitrogen in bone collagen, and of carbon and oxygen in the bone's structural carbonate of human skeletons from burial sites dated to the Linear Pottery Culture, the middle Neolithic, the Corded Ware and the Bell Beaker Culture revealed differences in the dietary behaviour between 5500 until 3000 BC, and between 3000 until 2000 BC, respectively. In late Neolithic times, meat procurement appears improved and the dietary spectrum as such broadened, evidencing a more secured and increasingly flexible subsistence strategy. Oxygen isotope ratios of the structural carbonate proved to be reliable climatic indicators and may be helpful in the dating of archaeological sites.
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