Related Experiment Video
Updated: Aug 9, 2026
Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
High-throughput genotyping with infrared fluorescence allele specific hybridization (iFLASH): a simple, reliable and
Thomas M van Himbergen1, Hieronymus A M Voorbij, Arjan D Barendrecht
1Research Laboratory of the Department of Clinical Chemistry, University Medical Center Utrecht, Utrecht, The Netherlands. T.M.vanHimbergen@azu.nl
Objectives:
To develop and validate a novel genotyping approach, named infrared Fluorescence Allele Specific Hybridization (iFLASH), which combines the principles of allele specific oligonucleotide (ASO) hybridization with the advanced possibilities of infrared imaging.
Design And Methods:
As an example, we genotyped the 55L > M and the 192Q > R common genetic variants of the paraoxonase-1 gene in 92 DNA samples using the iFLASH technique, and validated the outcomes with the restriction fragment length polymorphism (RFLP) and TAQman genotyping assays.
Results:
There was a 100 percent agreement in genotype outcome among the three methods.
Conclusions:
Although we found complete unity in genotype outcome, the iFLASH assay has essential advantages over the RFLP and TAQman genotyping assays. First, the iFLASH technique is capable of handling up to 1536 samples per assay, which makes it a suitable technique for high-throughput genotyping. Secondly, because the costs per assay are lower, high-throughput genotyping with iFLASH is affordable.
