Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix

Area of Science:

• Electrochemistry
• Materials Science
• Biotechnology

Background:

• Phenol detection is crucial for environmental monitoring due to its toxicity.
• Developing sensitive and stable biosensors is essential for accurate phenol determination.
• Chitosan and laponite offer promising properties for biosensor fabrication.

Purpose of the Study:

• To develop a novel amperometric biosensor for phenol determination.
• To utilize a chitosan/laponite nanocomposite matrix for immobilizing tyrosinase.
• To evaluate the analytical performance and stability of the fabricated biosensor.

Main Methods:

• Fabrication of a glassy carbon electrode modified with chitosan/laponite nanocomposite.
• Immobilization of polyphenol oxidase (PPO) onto the modified electrode surface.
• Amperometric detection of phenol and optimization of experimental parameters.

Main Results:

• The biosensor demonstrated a high sensitivity of 674 mA M(-1)cm(-2) for catechol.
• The apparent Michaelis-Menten constant indicated good substrate affinity (0.16 mM).
• The biosensor retained 88% of its activity after 60 days, showing excellent long-term stability.

Conclusions:

• The chitosan/laponite nanocomposite provides an effective matrix for PPO immobilization.
• The developed biosensor exhibits excellent sensitivity, stability, and affinity for phenol detection.
• This novel biosensor strategy holds potential for practical phenol monitoring applications.