Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rtn1p is involved in structuring the cortical endoplasmic reticulum.

Johan-Owen De Craene1, Jeff Coleman, Paula Estrada de Martin

  • 1Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA.

Molecular Biology of the Cell
|April 21, 2006
PubMed
Summary

Researchers found that Rtn1p protein influences endoplasmic reticulum (ER) structure in yeast. Rtn1p helps form the reticular ER network, potentially by interacting with the exocyst complex.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Turbocharging synaptic transmission: 12 SNAREpins are required for rapid release of reconstituted synaptic vesicles.

Science advances·2026
Same author

Synaptic transmission: Munc13 assembles onto PI(4,5)P<sub>2</sub>-rich domains into trimers that cooperate to capture vesicles.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release.

Nature communications·2025
Same author

Highly stable planar asymmetric suspended membranes for investigating protein dynamics and membrane fusion.

Nature protocols·2025
Same author

Lithium fine tunes lipid membranes through phospholipid binding.

Scientific reports·2025
Same author

Anti-obesity potential of selected medicinal plants: A focused study on in vitro inhibitory effects on lipase, α-amylase and α-glucosidase enzymes.

Journal of ethnopharmacology·2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Yeast Genetics

Background:

  • The endoplasmic reticulum (ER) exists as both cisternal and reticular structures.
  • The specific proteins regulating ER morphology, particularly the formation of reticular networks, are not fully understood.

Purpose of the Study:

  • To identify yeast genes involved in altering ER morphology.
  • To investigate the function of Rtn proteins in ER structure and their potential interactions with other cellular components.

Main Methods:

  • Systematic genetic screening of yeast mutants for altered ER morphology.
  • Fluorescent protein tagging (Rtn1-green fluorescent protein) to visualize ER structure and protein localization.
  • Proteomic analysis to identify proteins interacting with Rtn1p.

Related Experiment Videos

  • Analysis of yeast mutants with altered Rtn1p expression or function.

    • Main Results:

    • A yeast mutant, rtn1Delta, displayed predominantly cisternal ER instead of the typical reticular structure.
    • Rtn1-green fluorescent protein localized to the reticular ER network at the cell cortex and connecting tubules.
    • Rtn1p was identified as interacting with the exocyst complex, specifically binding to the Sec6p subunit.
    • Overexpression of the Rtn1p hydrophilic loop impaired exocyst function, leading to secretory vesicle accumulation.

    Conclusions:

    • Rtn proteins, including Rtn1p, likely play a crucial role in directing the formation of reticulated ER structures.
    • The interaction between Rtn1p and the exocyst complex at the bud tip may be essential for establishing the cortical ER network in yeast buds.