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Purification and properties of DNA gyrase from Vibrio cholerae
1Indian Institute of Chemical Biology, Calcutta.
The Biochemical Journal
|December 15, 1991
Abstract:
The DNA gyrase was purified from Vibrio cholerae strain 569B. It appeared to be composed of two subunits of Mr 120,000 and 97,000 and had a great deal of similarity to the Escherichia coli gyrase. Unlike the E. coli enzyme, however, it could neither relax supercoiled DNA nor induce a cleavage of double-stranded DNA, under experimental conditions in which E. coli gyrase could do so.