Jove
Visualize
Contact Us

Related Experiment Videos

RFLP analysis for mitochondrial genome of CMS-rice.

Wei Huang1, Li Wang, Ping Yi

  • 1Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, China.

Yi Chuan Xue Bao = Acta Genetica Sinica
|April 22, 2006
PubMed
Summary

Restriction fragment length polymorphism (RFLP) revealed mitochondrial genome differences in cytoplasmic male sterility (CMS) rice lines. These findings offer molecular evidence for cytoplasmic heterogeneity and advance research into the CMS mechanism in rice.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic and genetic alterations of PTEN in hepatocellular carcinoma.

Hepatology research : the official journal of the Japan Society of Hepatology·2007
Same author

Non-sebaceous lymphadenoma of the salivary gland: case report with immunohistochemical investigation.

Virchows Archiv : an international journal of pathology·2007
Same author

[Enhancement of HSP-MUC1 antitumor activity by type C CpG-ODN BW005].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2007
Same author

Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutralizing antibody in golden Syrian hamsters.

Viral immunology·2007
Same author

Synergistic effect between components of mixtures of cationic amphipaths in transfection of primary endothelial cells.

Molecular pharmaceutics·2007
Same author

The insertion polymorphism in angiotensin-converting enzyme gene associated with the APOE epsilon 4 allele increases the risk of late-onset Alzheimer disease.

Journal of molecular neuroscience : MN·2007
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Agricultural Science
  • Genetics
  • Molecular Biology

Background:

  • Cytoplasmic male sterility (CMS) is a crucial trait in hybrid rice breeding.
  • Understanding the genetic basis of CMS is vital for improving hybrid seed production.
  • Mitochondrial genomes play a key role in CMS, but their diversity in rice requires further investigation.

Purpose of the Study:

  • To investigate mitochondrial genome diversity in different cytoplasmic male sterility (CMS) rice lines.
  • To identify molecular differences between sterile (A) and maintainer (B) lines within CMS rice.
  • To provide molecular evidence supporting the hypothesis of cytoplasmic heterogeneity in rice CMS.

Main Methods:

  • Restriction fragment length polymorphism (RFLP) analysis was employed.

Related Experiment Videos

  • Mitochondrial (mt) genomes of nine types of CMS rice were analyzed.
  • Comparisons were made between sterile (A) and maintainer (B) lines, including functional gene regions.

    • Main Results:

    • Significant differences were observed in the mitochondrial genomes between sterile (A) and maintainer (B) lines across nine CMS rice types.
    • Mitochondrial genomic disparities were detected in numerous functional gene regions between A and B lines.
    • Even rice materials with identical nuclear backgrounds exhibited variations in their mitochondrial DNA (mtDNA).

    Conclusions:

    • The study provides robust molecular evidence for cytoplasmic heterogeneity in CMS rice.
    • The observed mitochondrial DNA variations contribute to understanding the complex mechanism of cytoplasmic male sterility in rice.
    • These findings have implications for breeding strategies aimed at developing novel CMS systems and improving hybrid rice varieties.