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Updated: Aug 9, 2026
Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Simultaneous compensation for spatial and temporal dispersion of acousto-optical deflectors for two-dimensional
Shaoqun Zeng1, Xiaohua Lv, Chen Zhan
1National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China. sqzeng@mail.hust.edu.cn
Abstract:
The dispersive nature of the acousto-optical deflector (AOD) presents a challenge to applications of two sequential orthogonal AODs (a two-dimensional AOD) as XY scanners in multiphoton microscopy. Introducing a prism before the two-dimensional (2D) AOD allows both temporal and spatial dispersion to be compensated for simultaneously. A 90 fs laser pulse was broadened to 572 fs without compensation, and 143 fs with compensation. The ratio of long axis to short axis of the exiting laser beam spot was 3.50 without compensation and 1.14 with compensation. The insertion loss was 37%. Two-photon fluorescence microscopy used the compensated 2D AOD scanner to image a fluorescent microsphere, which improves signal intensity -15-fold compared with the uncompensated scanner.
