Related Experiment Video

Updated: Aug 9, 2026

Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
03:33

Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology

Published on: May 23, 2025

Stool antigen tests for the detection of Helicobacter pylori in children

Kaija-Leena Kolho1, Timo Klemola, Antti Koivusalo

  • 1Hospital for Children and Adolescents, Box 281, FIN-00029 Helsinki, Finland. kaija-leena.kolho@helsinki.fi

Diagnostic Microbiology and Infectious Disease
|April 22, 2006
PubMed

Insights

Stool antigen tests accurately detect Helicobacter pylori in children. The HpStar assay showed high sensitivity and specificity, performing excellently in young patients, even with rectal swab samples.

Area of Science:

  • Pediatric Gastroenterology
  • Microbiology
  • Diagnostic Testing

Background:

  • Helicobacter pylori infection is a significant global health concern, particularly in children.
  • Accurate and non-invasive diagnostic methods are crucial for managing H. pylori in pediatric populations.

Purpose of the Study:

  • To evaluate the diagnostic performance of the HpStar stool antigen test for H. pylori detection in children.
  • To compare the HpStar test with other commercially available stool antigen tests and biopsy-based methods.

Main Methods:

  • Stool specimens from 102 children were analyzed using the HpStar monoclonal antibody-based enzyme immunoassay.
  • Rectal cotton swab specimens were used in 28 cases during endoscopy.
  • Parallel testing was performed with Premium Platinum HpSA and ImmunoCardSTAT! rapid test for a subset of specimens.

Main Results:

  • The HpStar test demonstrated high sensitivity (0.95) and specificity (0.90) compared to biopsy-based methods.
  • Rectal cotton swab specimens yielded accurate results in 24 out of 28 cases.
  • The HpStar and HpSA tests achieved 98% accuracy, while ImmunoCardSTAT! had 96% accuracy, indicating excellent performance in young children.

Conclusions:

  • The HpStar stool antigen test is a reliable and accurate method for diagnosing H. pylori infection in children.
  • Stool antigen testing, including the HpStar assay, offers an excellent diagnostic option for pediatric H. pylori screening and management.

More Related Videos

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis04:56

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Published on: May 16, 2025

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance06:40

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

Related Concept Videos

Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies01:28

Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies

Peptic ulcer disease (PUD) presents with diverse symptoms depending on the location and severity of the ulcer. Clinical manifestations of peptic ulcer include dull pain and a burning sensation in the mid-epigastric region.
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
Serum Laboratory Studies, Stool Test, Breath Test01:30

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
Peptic Ulcer01:27

Peptic Ulcer

Peptic ulcers are erosive lesions of the gastric or duodenal lining, most commonly caused by Helicobacter pylori infection. This Gram-negative, helical bacterium has adapted to survive the stomach’s acidic environment by producing urease, which converts urea into ammonia and carbon dioxide. The ammonia neutralizes gastric acid in the bacterium’s immediate environment, allowing colonization of the gastric mucosa. H. pylori attaches to mucus-secreting epithelial cells, penetrates the mucus...
Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...
Microbiota of the Stomach and Small Intestine01:27

Microbiota of the Stomach and Small Intestine

The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...