Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel approach to enterovirus typing.

S H Lee1, J E Boulilier, M A MacDonald

  • 1Department of Microbiology, Dalhousie University, Victoria General Hospital, Halifax, Nova Scotia, Canada.

Journal of Medical Virology
|October 1, 1991
PubMed
Summary

A new agarose overlay method rapidly and efficiently types enteroviruses, including polioviruses, coxsackieviruses, and echoviruses, by observing cytopathic effect inhibition. This technique simplifies enterovirus identification in laboratories.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atomic versus molecular Auger decay in CH<sub>2</sub>Cl<sub>2</sub> and CD<sub>2</sub>Cl<sub>2</sub> molecules.

The Journal of chemical physics·2018
Same author

Structure and formation of highly luminescent protein-stabilized gold clusters.

Chemical science·2018
Same author

Fragmentation of Valence and Core-Shell (Cl 2p) Excited C<sub>2</sub>Cl<sub>4</sub> Molecule.

The journal of physical chemistry. A·2017
Same author

Valence and Ionic Lowest-Lying Electronic States of Isobutyl Formate Studied by High-Resolution Vacuum Ultraviolet Photoabsorption, Photoelectron Spectroscopy, and Ab Initio Calculations.

The journal of physical chemistry. A·2015
Same author

The rapid emergence of high level gentamicin resistance in enterococci.

The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012
Same author

Comparative activity of daptomycin and teicoplanin against enterococci isolated from blood and urine.

The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012

Area of Science:

  • Virology
  • Microbiology
  • Immunology

Background:

  • Enteroviruses are a significant public health concern, necessitating accurate and rapid identification.
  • Conventional methods for enterovirus typing can be time-consuming and labor-intensive.
  • Cytopathic effect (CPE) is a key indicator of viral infection, but its direct use for typing requires refinement.

Purpose of the Study:

  • To develop and validate a novel, rapid, and efficient method for typing enteroviruses based on CPE inhibition.
  • To assess the accuracy of the new method compared to conventional micro-neutralization techniques.
  • To provide laboratories with a simplified and robust tool for enterovirus identification.

Main Methods:

  • Preparation of rhesus monkey kidney cell monolayers with an agarose overlay.

Related Experiment Videos

  • Creation of wells within the agarose for antiserum and virus addition.
  • Incubation and observation of cytopathic effect (CPE) inhibition following the addition of enterovirus isolates and specific antisera.
  • Confirmation of typing results using a standard micro-neutralization assay.

    • Main Results:

    • Successfully typed 51 enterovirus isolates, including 21 polioviruses, 9 coxsackieviruses, and 21 echoviruses.
    • Achieved accurate identification of all tested enterovirus types.
    • Demonstrated that the method tolerates a 1,000-fold fluctuation in virus concentration.
    • Confirmed the method's simplicity and lack of requirement for preliminary virus titration.

    Conclusions:

    • The novel agarose overlay method provides a simple, rapid, and efficient means for typing enteroviruses.
    • This technique leverages the diffusion of enteroviruses and antibodies through agarose for CPE inhibition.
    • The method offers a valuable alternative for diagnostic laboratories requiring accurate enterovirus identification.