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Perivascular cells along venules upregulate NG2 expression during microvascular remodeling.
Walter L Murfee1, Michael R Rehorn, Shayn M Peirce
1Department of Biomedical Engineering, University of Virginia, Health System, Charlottesville, Virginia 22908, USA.
Microcirculation (New York, N.Y. : 1994)
|April 22, 2006
Summary
Neuron-glial antigen 2 (NG2) proteoglycan is transiently upregulated along venules during microvascular remodeling in rats. This suggests NG2 marks activated venules during tissue repair and regeneration processes.
Area of Science:
- Vascular biology
- Proteoglycan research
- Tissue remodeling
Background:
- Neuron-glial antigen 2 (NG2) is typically absent from venules in quiescent rat mesenteric microvasculature.
- Understanding NG2 expression dynamics is crucial for studying microvascular network changes.
Purpose of the Study:
- To investigate the spatial distribution of NG2 during active microvascular remodeling.
- To characterize NG2 expression changes in adult rat mesenteric microvascular networks.
Main Methods:
- Adult female Sprague-Dawley rats were used, with tissues harvested at 1, 3, and 5 days poststimulation.
- Double immunolabeling for NG2 and CD31 (endothelial cell marker) was performed.
Main Results:
- NG2 expression was detected on 27% of venules at 1 day and 59% at 3 days poststimulation.
- By day 5, NG2 expression on venules decreased to 18%, returning to quiescent levels.
- This transient upregulation occurred along draining venules of specific sizes (13-59 microm).
Conclusions:
- NG2 proteoglycan expression is transiently upregulated along venules during microvascular remodeling.
- NG2 serves as a marker for activated venules in remodeling microvascular networks.