Replication dependent and cell specific activation of the polyomavirus early promoter

The relationship of viral DNA replication to the activation of viral gene expression is usually considered with respect to late genes. In this report we examine the replication activation of the polyomavirus early promoter. Using origin active and inactive mutants to drive luciferase gene expression from the polyomavirus early promoter, we show that the early promoter is also subjected to a replication dependent activation. The degree of activation can be up to a hundred fold greater than that seen without replication and is about 13 fold on a per template basis. This replication based activation is, however, cell type dependent and was seen in FOP cells but not in 3T6 cells. Analysis of the requirements of cis acting DNA show that these enhancer elements affect early transcription predominantly through the activation of replication, although some replication independent stimulation can also be seen. The implications of this result for the regulation of polyomavirus early gene regulation are considered.