Molecular comparison of VP3 from bluetongue and epizootic hemorrhagic disease viruses

The complete nucleic acid sequence of gene 3 from epizootic hemorrhagic disease of deer (EHD) virus serotype 1 was determined. The 2768 bp sequence encodes a single protein that contains 899 amino acids and has a molecular weight of 103 kDa. The predicted protein sequence has 94.7% identity with EHD virus serotype 2 and greater than 77% identity with the related bluetongue viruses serotypes 1, 10 and 17 VP3 proteins. The relevance of these data to studies of recombinant DNA diagnostics and genetic relatedness is discussed.