[Nosocomial infection by multidrug-resistant Pseudomonas Aeruginosa (MDRP) presumably spread a radiation technician]
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Updated: Aug 9, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
Shin Nihonyanagi1, Yasuyosi Hirata, Tooru Akabosi
1Department of Medical Laboratory, Kitasato University Hospital.
Abstract:
Isolated of multidrug resistance Pseudomonas aeruginosa (MDRP) that the receptivity pattern of the antimicrobial suscepti respectively resembled isolated from clinical specimens (sputum) in two patients of each internal medicine ward in Kitasato University East Hospital for two days from September 18 and 20, 2004. Both of bacteria were formed small colonies of a smooth-type on dollargalluskey improvement-type BTB agar plates, and the judgment of ClassB (metallo)-beta-lactamase by biochemical properties and disk diffusion method sodium mercaoto-acetic acid (SMA) was mutually corresponding. Moreover, it was same serotype C according to the serotype, and it was confirmed that it was the same bacterial strain from the molecular epidemiology analysis by Random amplified polymorphic DNA polymerase chain reaction (Random amplified polymorphic DNA polymerase chain reaction: RAPD). From the investigation of clinical backgrounds of two patients who isolated bacterial strains, September 18, 2004. 10 : 20 a.m., and 10 : 40 a.m., other chances that can become with contact infection in this hospital, except conducted X-Ray or roentgenograph of the chest and abdomen of Portable X-ray device continuously done by one radiation technician was not seen. Because it had turned out that a radiation technician who had taken charge had been neglecting the hand washing at the time of each X-Ray or roentgenograph, it was guessed the case with nosocomial infection by contact infection occurred via specific radiation technician.
Insights
Multidrug-resistant Pseudomonas aeruginosa (MDRP) strains were identified in hospitalized patients. A radiation technician
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Hospital Epidemiology
Background:
- Multidrug-resistant Pseudomonas aeruginosa (MDRP) poses a significant threat in healthcare settings.
- Identifying the transmission routes of MDRP is crucial for infection control.
Purpose of the Study:
- To investigate the source and transmission of MDRP isolated from two patients in a hospital.
- To determine if the isolated MDRP strains were genetically identical.
Main Methods:
- Antimicrobial susceptibility testing and biochemical characterization.
- Serotyping and Random Amplified Polymorphic DNA (RAPD) analysis for molecular epidemiology.
- Review of patient clinical backgrounds and hospital procedures.
Main Results:
- Two MDRP strains isolated from patients were identical by serotype and RAPD analysis.
- The strains exhibited Class B (metallo)-beta-lactamase activity.
- A single radiation technician performed X-rays on both patients shortly after each other.
Conclusions:
- The MDRP strains were likely transmitted via contact infection.
- The transmission was suspected to occur through a radiation technician who neglected hand hygiene.
- This highlights the importance of strict adherence to infection control protocols in hospitals.
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