Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sheep-urine-induced changes in soil microbial community structure.

Naoise Nunan1, Brajesh Singh, Eileen Reid

  • 1Biomathematics and Statistics Scotland, Scottish Crop Research Institute, Dundee, UK. nunan@grignon.inra.fr

FEMS Microbiology Ecology
|April 25, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanoclay-based dsRNA delivery: a novel approach to control Globodera rostochiensis.

Molecular biology reports·2026
Same author

Aeroponic root leachate (ARL)-induced hatching as a sustainable strategy for the management of Globodera rostochiensis in potato (Solanum tuberosum L.).

Scientific reports·2026
Same author

Precision pest management: Genome editing tools, specifically CRISPR/Cas9 and future prospects.

Pesticide biochemistry and physiology·2026
Same author

CRISPR-Cas9 mediated editing of <i>starch branching enzyme</i>, <i>SBE2</i> gene in potato for enhanced resistant starch for health benefits.

Frontiers in genome editing·2025
Same author

Enumeration of Ki67 proliferation index by an algorithm-based digital image analysis in breast carcinoma.

Medical journal, Armed Forces India·2025
Same author

Launching the IUCN Microbial Conservation Specialist Group as a global safeguard for microbial biodiversity.

Nature microbiology·2025

Synthetic urine alters soil microbial community structure, shifting towards branched fatty acids and impacting carbon use, though overall community stability remains largely unaffected.

Area of Science:

  • Soil microbiology
  • Environmental science
  • Ecology

Background:

  • Soil microbial communities are crucial for nutrient cycling, particularly in unimproved soils.
  • Grazed pastures experience nutrient heterogeneity due to animal waste, like sheep urine.
  • Understanding urine's impact on soil microbes is key for pasture management.

Purpose of the Study:

  • To investigate the effects of synthetic urine on soil microbial community structure.
  • To assess changes using physiological, biochemical, and molecular methods.
  • To determine the extent of microbial community shifts induced by urine application.

Main Methods:

  • Community Level Physiological Profiling (CLPP) for carbon source utilization.
  • Phospholipid Fatty Acid (PLFA) analysis for microbial biomass and community structure.

Related Experiment Videos

  • Denaturing Gradient Gel Electrophoresis (DGGE) for molecular fingerprinting of microbial DNA.

    • Main Results:

    • Synthetic urine did not significantly alter total microbial biomass (PLFA).
    • Significant shifts in microbial community structure were detected by PLFA and DGGE.
    • Urine treatment induced a preference for rhizosphere-C utilization and a shift towards branched fatty acids.

    Conclusions:

    • Synthetic urine significantly alters soil microbial community structure and function.
    • Despite shifts, the overall soil microbial community structure remains relatively stable.
    • These changes are confined to a small proportion of the microbial community.