Atomic force microscopy as an innovative tool for nanoanalysis of native stratum corneum

Area of Science:

• Biophysics
• Dermatology
• Materials Science

Background:

• Stratum corneum (SC) structure is crucial for skin barrier function.
• Nanoscale analysis of SC is essential for understanding skin aging.
• Existing methods have limitations in high-resolution SC structural analysis.

Purpose of the Study:

• To introduce a novel method combining atomic force microscopy (AFM) and tape stripping for nanoscale SC structure analysis.
• To investigate topographical and structural differences in SC between aged and young skin.
• To provide new insights into the structure, function, and morphodynamics of SC.

Main Methods:

• High-resolution atomic force microscopy (AFM) for nanoscale imaging.
• Tape stripping technique to obtain SC samples.
• Comparative analysis of SC from aged and young skin.

Main Results:

• Achieved vertical resolution of approximately 10 nm for SC topography.
• Observed significant topographical and structural differences in SC between aged and young skin.
• Aged skin SC exhibited increased single-cell surface area, prominent intercellular gaps, and enhanced cell surface roughness.

Conclusions:

• The combined AFM and tape stripping method is effective for nanoscale SC structure analysis.
• Significant structural alterations in SC are associated with skin aging.
• This approach offers valuable insights for dermatological research on SC structure, function, and morphodynamics.