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New polar-embedded stationary phase for surfactant analysis
Xiaodong Liu1, Christopher A Pohl, Joachim Weiss
1Dionex Corporation, Sunnyvale, CA 94088, USA.
Journal of Chromatography. A
|April 25, 2006
Summary
A new HPLC column enables simultaneous analysis of anionic, non-ionic, and cationic surfactants. This method simplifies surfactant characterization and quantitation in complex mixtures using a single mobile phase.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Surfactant analysis is complex due to diverse types, raw material composition, and sample matrices.
- High-performance liquid chromatography (HPLC) is crucial for surfactant characterization and quantitation.
- Existing HPLC columns lack optimal selectivity for simultaneous analysis of all surfactant types.
Purpose of the Study:
- To develop a novel HPLC stationary phase for simultaneous analysis of anionic, non-ionic, and cationic surfactants.
- To establish a simple, volatile mobile phase system for comprehensive surfactant analysis.
Main Methods:
- Utilized a new polar-embedded stationary phase in HPLC.
- Employed a mobile phase system with ammonium acetate buffer and acetonitrile.
- Used evaporative light-scattering detection (ELSD) for detection.
Main Results:
- Achieved simultaneous analysis of anionic, non-ionic, and cationic surfactants.
- Demonstrated ideal selectivity, excellent peak shapes (especially for cationic surfactants), and improved resolution for ethoxylated surfactant oligomers.
- Showcased compatibility with highly aqueous mobile phases.
Conclusions:
- The new polar-embedded column provides optimal selectivity for diverse surfactant types.
- This method facilitates quality assurance of individual surfactants and analysis of complex formulations.
- The developed HPLC approach simplifies simultaneous surfactant analysis.